4TYI
Structural analysis of the human Fibroblast Growth Factor Receptor 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.900, 65.900, 182.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.970 - 3.400 |
| R-factor | 0.28146 |
| Rwork | 0.277 |
| R-free | 0.33994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.025 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.970 | 3.690 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.087 | 0.831 |
| Number of reflections | 5806 | |
| <I/σ(I)> | 0.104 | |
| Completeness [%] | 96.4 | 96.8 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M Citric acid pH 4.0 1.6 M Ammonium sulfate final pH: 5 |
