4TWA
Crystal Structure of Prolyl-tRNA Synthetase (PRS) from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-04-23 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.240, 104.106, 111.420 |
| Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
| Resolution | 38.790 - 3.000 |
| R-factor | 0.1753 |
| Rwork | 0.172 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k86 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.227 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.159 | 0.689 |
| Number of reflections | 30738 | |
| <I/σ(I)> | 12.4 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.8 M lithium sulphate monohydrate and 0.1 M sodium acetate |
