4TVS

LAP1(aa356-583), H.sapiens, bound to VHH-BS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2548
Spacegroup name	P 1 21 1
Unit cell lengths	69.786, 74.021, 85.425
Unit cell angles	90.00, 108.79, 90.00

Refinement procedure

Resolution	66.068 - 1.600
R-factor	0.1824
Rwork	0.181
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bzq
RMSD bond length	0.019
RMSD bond angle	1.656
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.100	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.091	1.412
Number of reflections	105565
<I/σ(I)>	12.5	1.1
Completeness [%]	97.6	94.3
Redundancy	5.9	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	PEG 3350, 20% 0.1M BIS-TRIS 0.2M ammonium sulfate