4TUY

Tubulin-Rhizoxin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-03
Detector	PSI PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	104.310, 156.920, 181.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.700 - 2.100
R-factor	0.186
Rwork	0.184
R-free	0.21700
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4i4t
RMSD bond length	0.002
RMSD bond angle	0.658
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.400	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.165
Number of reflections	173215
<I/σ(I)>	16.9	1.3
Completeness [%]	100.0	100
Redundancy	13.6	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	6% PEG, 16% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1M MES/0.1M imidazole