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4TUX

drosophila stem-loop binding protein complexed with histone mRNA stem-loop

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-BM
Synchrotron siteAPS
Beamline22-BM
Temperature [K]100
Detector technologyCCD
Collection date2012-04-11
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)1.000
Spacegroup nameP 43 21 2
Unit cell lengths74.951, 74.951, 173.479
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution37.538 - 3.080
R-factor0.2612
Rwork0.255
R-free0.31720
RMSD bond length0.016
RMSD bond angle1.380
Refinement softwarePHENIX ((phenix.refine: 1.8_1069))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0003.150
High resolution limit [Å]3.0803.080
Rmerge0.0990.500
Number of reflections9127
<I/σ(I)>33.7
Completeness [%]93.460.4
Redundancy12.36.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.527716% (wt/vol) PEG 1000, 0.2 M Ca(Ac)2, 50 mM cacodylic acid, pH 6.5

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