4TUW
drosophila stem-loop binding protein complexed with histone mRNA stem-loop, phospho mimic of TPNK and C-terminal region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.810, 74.810, 161.285 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.733 - 2.902 |
R-factor | 0.2194 |
Rwork | 0.214 |
R-free | 0.26310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.168 |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.470 | |
Number of reflections | 10448 | |
<I/σ(I)> | 13.6 | 3 |
Completeness [%] | 97.6 | 81.6 |
Redundancy | 7.2 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 16% (wt/vol) PEG3350, 0.2 M Ca(Ac)2, 50 mM cacodylate acid, pH 6.5 |