4TUS
Human DNA polymerase beta inserting dCMPNPP opposite the 5'G of cisplatin crosslinked Gs (Pt-GG2) WITH MANGANESE IN THE ACTIVE SITE.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-05-31 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.878, 78.417, 54.803 |
| Unit cell angles | 90.00, 112.80, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.420 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.24300 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.120 |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.420 |
| Number of reflections | 16369 |
| <I/σ(I)> | 17.7 |
| Completeness [%] | 99.9 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole |
