4TUR

Human DNA polymerase beta inserting dCMPNPP opposite the 5'G of cisplatin crosslinked Gs (Pt-GG2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-05-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	54.785, 79.335, 54.786
Unit cell angles	90.00, 107.81, 90.00

Refinement procedure

Resolution	19.834 - 2.169
R-factor	0.2046
Rwork	0.202
R-free	0.25570
RMSD bond length	0.004
RMSD bond angle	1.130
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.169
Number of reflections	23644
<I/σ(I)>	22.8
Completeness [%]	100.0
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole