4TUP
Structure of human DNA polymerase beta complexed with GG as the template (GG0b) in a 1-nucleotide gapped DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.354, 79.257, 54.927 |
| Unit cell angles | 90.00, 105.42, 90.00 |
Refinement procedure
| Resolution | 19.814 - 1.800 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.23600 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.822 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 41437 |
| <I/σ(I)> | 26.4 |
| Completeness [%] | 100.0 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 18% PEG3400, and 350 mM sodium acetate in 50 mM imidazole |
