4TUJ

Crystal structure of monoclonal antibody against neuroblastoma associated antigen.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	80
Detector technology	IMAGE PLATE
Collection date	2013-06-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	43.380, 69.820, 78.000
Unit cell angles	111.45, 101.14, 90.35

Refinement procedure

Resolution	24.777 - 1.890
R-factor	0.1816
Rwork	0.179
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f8t
RMSD bond length	0.012
RMSD bond angle	1.388
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.840	1.990
High resolution limit [Å]	1.890	1.890
Rmerge	0.069	0.548
Number of reflections	60908
<I/σ(I)>	9.66	1.85
Completeness [%]	90.9	84.54
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Bis-Tris containing PEG 5000