4TUD
Crystal structure of ASL-SufJ bound to Codon ACC-C on the Ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-22 |
| Detector | RAYONIX MX300HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.464, 450.700, 621.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.847 - 3.600 |
| R-factor | 0.2066 |
| Rwork | 0.205 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3I8F 3I8G 3I8H 3I8I |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.983 |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.700 | |
| High resolution limit [Å] | 3.600 | 35.000 | 3.600 |
| Rmerge | 0.187 | 0.056 | 0.908 |
| Rmeas | 0.208 | 0.070 | 1.011 |
| Total number of observations | 3587145 | ||
| Number of reflections | 675413 | 441 | 52893 |
| <I/σ(I)> | 7.84 | 4.96 | 1.93 |
| Completeness [%] | 99.9 | 50.9 | 99.8 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl |
