4TTW

N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p K105C F145C (NN18)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-10-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	C 1 2 1
Unit cell lengths	98.040, 29.300, 63.720
Unit cell angles	90.00, 126.99, 90.00

Refinement procedure

Resolution	20.784 - 1.200
R-factor	0.1641
Rwork	0.163
R-free	0.18860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q0y
RMSD bond length	0.010
RMSD bond angle	1.340
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.898	1.230
High resolution limit [Å]	1.200	1.200
Rmerge		1.514
Number of reflections	45556
<I/σ(I)>	16.2	1.35
Completeness [%]	99.0	95.5
Redundancy	6.4	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	293	PEG3350, MES-NaOH