4TT8

Crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase (wild-type) complex with 10-formyl-5,8-dideazafolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	110
Detector technology	CCD
Collection date	2011-05-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	104.075, 52.694, 60.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.347 - 2.301
R-factor	0.1819
Rwork	0.179
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ts4
RMSD bond length	0.004
RMSD bond angle	0.968
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300
Number of reflections	15370
<I/σ(I)>	15.8
Completeness [%]	100.0
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	Bis-Tris (0.1~0.2 M, pH 5.5) and PEG3350 (25~29%, w/v)