4TT3
The Pathway of Binding of the Intrinsically Disordered Mitochondrial Inhibitor Protein to F1-ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-07 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.385, 154.545, 272.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 136.020 - 3.210 |
| R-factor | 0.2443 |
| Rwork | 0.242 |
| R-free | 0.28340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v7q |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.732 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.120 | 3.280 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Rmerge | 0.078 | |
| Number of reflections | 73010 | |
| <I/σ(I)> | 7.6 | 2.3 |
| Completeness [%] | 97.5 | 99.1 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.2 | 295 | PEG 4000, Tris-HCl, magnesium chloride, EDTA, ATP, NaCl, spermidine |
