4TSV

HIGH RESOLUTION CRYSTAL STRUCTURE OF A HUMAN TNF-ALPHA MUTANT

Experimental procedure

Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-12
Detector	MACSCIENCE
Spacegroup name	H 3
Unit cell lengths	66.720, 66.720, 85.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.2
Rwork	0.200
R-free	0.26200
RMSD bond length	0.015
RMSD bond angle	2.177
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.01)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.800
Rmerge	0.040 *
Number of reflections	13102
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8	24 *	1.4 M SODIUM FORMATE, pH 6.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop	sodium formate	2.8 (M)
4	1	reservoir	sodium formate	2.8 (M)