4TSB
Structure of a lysozyme antibody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.600, 99.910, 75.090 |
| Unit cell angles | 90.00, 110.12, 90.00 |
Refinement procedure
| Resolution | 34.890 - 1.950 |
| R-factor | 0.1954 |
| Rwork | 0.194 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.890 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.391 | |
| Number of reflections | 34734 | |
| <I/σ(I)> | 12.2 | 2.2 |
| Completeness [%] | 85.4 | 39.1 |
| Redundancy | 4.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM PCPT pH 5.5, 200mM MgCl2, 22 % w/v PEG 3350 |
