4TS6

Crystal structure of the RIM C2A domain from Drosophila.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999
Spacegroup name	P 21 21 21
Unit cell lengths	32.080, 38.700, 131.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.680 - 1.920
R-factor	0.186
Rwork	0.184
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bwq
RMSD bond length	0.007
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	Aimless (3.11)
Phasing software	MrBUMP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.680		1.970
High resolution limit [Å]	1.920	8.590	1.920
Rmerge	0.057	0.025	0.643
Rmeas	0.066	0.029	0.751
Total number of observations	90891
Number of reflections	13188	269	1756
<I/σ(I)>	12.39	28.68	1.97
Completeness [%]	99.6	95.7	99.6
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M tri-lithium citrate, 20% w/v PEG 3350