4TS4

Crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase (wild-type) from zebrafish

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	110
Detector technology	CCD
Collection date	2010-02-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	104.665, 53.878, 60.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.025 - 1.750
R-factor	0.1813
Rwork	0.180
R-free	0.20070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s3i
RMSD bond length	0.007
RMSD bond angle	1.302
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge		0.440
Number of reflections	35347
<I/σ(I)>	33.8	4
Completeness [%]	99.9	99.9
Redundancy	6.9	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	Bis-Tris (0.1~0.2 M, pH 5.5) and PEG3350 (25~29%, w/v)