4TS0

Crystal structure of the Spinach RNA aptamer in complex with DFHBI, barium ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.5498
Spacegroup name	P 21 21 21
Unit cell lengths	39.760, 49.500, 186.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.2076
Rwork	0.206
R-free	0.23870
RMSD bond length	0.008
RMSD bond angle	1.958
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall
Low resolution limit [Å]	93.100
High resolution limit [Å]	2.500
Number of reflections	9193
<I/σ(I)>	19
Completeness [%]	99.0
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350, 0.25M NaCl, 0.1M succinate-K pH 7.0, 15% sucrose