4TRU
Crystal structure of Q24A Q25A mutant of human macrophage migration inhibitory factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-06-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.513, 67.578, 88.569 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.760 - 1.810 |
| R-factor | 0.1598 |
| Rwork | 0.158 |
| R-free | 0.19050 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.825 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.840 |
| High resolution limit [Å] | 1.810 | 4.910 | 1.810 |
| Rmerge | 0.052 | 0.045 | 0.209 |
| Total number of observations | 174375 | ||
| Number of reflections | 37251 | ||
| <I/σ(I)> | 16.2 | ||
| Completeness [%] | 98.8 | 92.6 | 94.5 |
| Redundancy | 4.7 | 4.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 2 M ammonium Sulphate, 3% 2-propanol,20 mM Tris.HCl pH 7.5 |
