4TRH
The Legionella effector SidC defines a unique family of ubiquitin ligases important for bacterial phagosomal remodeling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.230, 132.685, 171.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.900 - 2.030 |
| R-factor | 0.21499 |
| Rwork | 0.213 |
| R-free | 0.24929 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.765 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.900 |
| High resolution limit [Å] | 2.030 |
| Number of reflections | 90100 |
| <I/σ(I)> | 25 |
| Completeness [%] | 95.2 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1 M cacodylate pH 5.6, 7.5% PEG6000, and 10 mM DTT |
