4TRB

Sulfolobus solfataricus adenine phosphoribosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	122
Detector technology	CCD
Collection date	2007-02-01
Detector	MARRESEARCH
Wavelength(s)	0.93100
Spacegroup name	P 61
Unit cell lengths	125.628, 125.628, 69.707
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.721 - 2.400
R-factor	0.2306
Rwork	0.228
R-free	0.28650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1NUL and 1VDM
RMSD bond length	0.008
RMSD bond angle	0.985
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400
Number of reflections	24914
<I/σ(I)>	23.8
Completeness [%]	100.0
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein: 6.2 mg/mL, 25 mM TRIS pH 7.6, 0.1 mM EDTA, Buffer: 0.1 M MES pH 6.5, Precipitant: 30 % Jeffamine M-600