4TQU
Crystal structure of a bacterial ABC transporter involved in the import of the acidic polysaccharide alginate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.367, 134.177, 273.798 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.638 - 3.204 |
R-factor | 0.2393 |
Rwork | 0.237 |
R-free | 0.28160 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.057 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.200 |
Number of reflections | 43105 |
<I/σ(I)> | 23.9 |
Completeness [%] | 97.6 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3000, N-(2-acetamido)iminodiacetic acid NaCl |