4TQE

Structure of tau peptide in complex with Tau5 antibody Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2669
Spacegroup name	C 1 2 1
Unit cell lengths	124.865, 59.732, 71.041
Unit cell angles	90.00, 112.76, 90.00

Refinement procedure

Resolution	65.510 - 1.600
R-factor	0.1535
Rwork	0.151
R-free	0.19715
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	12e8
RMSD bond length	0.009
RMSD bond angle	1.370
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.510	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.056	0.858
Number of reflections	60326
<I/σ(I)>	10.71	1.06
Completeness [%]	94.6	77.8
Redundancy	3.18	2.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2M lithium sulphate, 30% PEG 4000, 0.1M Tris pH 8.5