4TPW
The co-complex structure of the translation initiation factor eIF4E with the inhibitor 4EGI-1 reveals an allosteric mechanism for dissociating eIF4G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2011-07-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.340, 73.200, 65.720 |
| Unit cell angles | 90.00, 106.25, 90.00 |
Refinement procedure
| Resolution | 19.666 - 1.500 |
| R-factor | 0.1725 |
| Rwork | 0.172 |
| R-free | 0.19280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ej1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.812 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 54446 | |
| <I/σ(I)> | 21.51 | |
| Completeness [%] | 95.2 | 96 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | PEG 4000 10-20%, 100mM MES pH 6.5, 10% IPN |
