4TPG
Selectivity mechanism of a bacterial homologue of the human drug peptide transporters PepT1 and PepT2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-04-20 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.890, 108.090, 206.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 4.010 - 3.910 |
| R-factor | 0.2622 |
| Rwork | 0.261 |
| R-free | 0.29030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lep |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.851 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 4.010 | 4.010 |
| High resolution limit [Å] | 3.910 | 3.910 |
| Number of reflections | 31871 | |
| <I/σ(I)> | 7.14 | 2.18 |
| Completeness [%] | 90.6 | 39.3 |
| Redundancy | 4.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 297 | 0.1 M phosphate citrate, 36% PEG300, 0.12 M ZnCl2 |
