4TOI

Crystal structure of E.coli ribosomal protein S2 in complex with N-terminal domain of S1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98
Spacegroup name	P 31 2 1
Unit cell lengths	87.280, 87.280, 94.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.790 - 2.300
R-factor	0.1668
Rwork	0.164
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 2qbf and 2oce
RMSD bond length	0.008
RMSD bond angle	1.090
Data reduction software	XDS (0.1.29)
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.790	37.790	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.175	0.071	1.106
Rpim	0.063	0.024	0.543
Total number of observations	151502	3479	7560
Number of reflections	18726
<I/σ(I)>	7.4	21.7	1.5
Completeness [%]	99.0	98.1	91.3
Redundancy	8.1	9.5	4.5
CC(1/2)	0.993	0.995	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	295.15	0.1 M HEPES-KOH, pH 7.4, 3 mM MgCl2, 7.5% w/v PEG 6000, 3% w/v 2-methyl-pentanediol-2,4, 100 mM KCl