4TNY
Structural basis of cellular dNTP regulation, SAMHD1-dGTP-dATP-dGTP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.253, 141.874, 98.333 |
| Unit cell angles | 90.00, 116.38, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.2152 |
| Rwork | 0.214 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bzb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.490 |
| Data reduction software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.153 | 0.099 | |
| Total number of observations | 252071 | ||
| Number of reflections | 66014 | ||
| <I/σ(I)> | 5.9 | ||
| Completeness [%] | 99.6 | 99.4 | 92.8 |
| Redundancy | 3.8 | 3.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | SPG buffer, PEG 1500 |
