4TN6
CK1d in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.487, 115.336, 61.533 |
| Unit cell angles | 90.00, 109.27, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.410 |
| R-factor | 0.1897 |
| Rwork | 0.187 |
| R-free | 0.23870 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | PDB 4KB8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.538 |
| Number of reflections | 28261 | |
| <I/σ(I)> | 13.76 | 1.57 |
| Completeness [%] | 98.3 | 85.9 |
| Redundancy | 3.36 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 296 | 0.1M NaAcetate, pH=5.6, 0.2 M Li2SO4, 14-17.5% PEG3350, 3-5.5% 1,3 propandiol |
