4TM3

Kutzneria sp. 744 ornithine N-hydroxylase, KtzI-FADox-Br

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-13
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	79.814, 151.943, 161.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.506 - 2.090
R-factor	0.2067
Rwork	0.205
R-free	0.23650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s5w
RMSD bond length	0.015
RMSD bond angle	1.657
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.130
High resolution limit [Å]	2.090	2.090
Number of reflections	117046
<I/σ(I)>	16.5	2.6
Completeness [%]	99.5	99.9
Redundancy	5.2	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.9-1.2 M sodium bromide, 22-25% PEG 3350, 0.1 M Bis-tris propane pH 7.5