4TM1

Kutzneria sp. 744 ornithine N-hydroxylase, KtzI-FADred-NADP+-Br

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-13
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	81.888, 151.711, 162.388
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.902 - 2.390
R-factor	0.2001
Rwork	0.199
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s5w
RMSD bond length	0.009
RMSD bond angle	1.289
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.390	2.390
Number of reflections	78161
<I/σ(I)>	14.7	2.4
Completeness [%]	96.8	98.1
Redundancy	5.5	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.2-1.4 M sodium bromide, 22-25% PEG 3350, 0.1 M Bis-tris propane pH 7.5