4TLM

Crystal structure of GluN1/GluN2B NMDA receptor, structure 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	203.490, 118.430, 226.590
Unit cell angles	90.00, 103.82, 90.00

Refinement procedure

Resolution	29.959 - 3.770
R-factor	0.2547
Rwork	0.253
R-free	0.29210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.438
Refinement software	PHENIX ((phenix.refine: dev_1723))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.200
High resolution limit [Å]	3.770
Number of reflections	44066
<I/σ(I)>	7.46
Completeness [%]	97.4
Redundancy	3.44

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	vapor diffusion, hanging drop, temperature 293K