4TLK

Crystal Structure of Human Transthyretin Ser85Pro/Glu92Pro Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-01
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	41.980, 85.240, 63.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.380 - 1.440
R-factor	0.1892
Rwork	0.189
R-free	0.20140
RMSD bond length	0.010
RMSD bond angle	1.050
Data scaling software	XSCALE
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	63.380
High resolution limit [Å]	1.440
Number of reflections	48638
<I/σ(I)>	13.34
Completeness [%]	99.0
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	20% (W/V) PEG-1000, 0.1M Imidazole, 0.2M Calcium Acetate