4TLH
Monoclinic Crystal Structure of EutL from Clostridium Perfringens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.440, 66.030, 79.380 |
Unit cell angles | 90.00, 108.27, 90.00 |
Refinement procedure
Resolution | 33.427 - 1.700 |
R-factor | 0.1644 |
Rwork | 0.163 |
R-free | 0.19510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.150 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1555)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.427 | 1.740 | |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.094 | 0.026 | 0.993 |
Rmeas | 0.110 | 0.031 | 1.159 |
Total number of observations | 235518 | ||
Number of reflections | 63757 | 744 | 4692 |
<I/σ(I)> | 11.02 | 33.63 | 1.62 |
Completeness [%] | 99.5 | 97.1 | 99.5 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 0.1M sodium potassium phosphate, 0.25 sodium chloride, 10% PEG 8000 |