4TL1
GCN4-p1 with mutation to 1-Aminocyclohexanecarboxylic acid at residue 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-12-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.322, 30.350, 28.019 |
| Unit cell angles | 90.00, 101.68, 90.00 |
Refinement procedure
| Resolution | 20.480 - 1.800 |
| R-factor | 0.1866 |
| Rwork | 0.185 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zta |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.106 |
| Phasing software | CrystalClear |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.480 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.176 |
| Number of reflections | 6386 | |
| <I/σ(I)> | 12.9 | 3 |
| Completeness [%] | 98.1 | |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M sodium citrate tribasic, 0.1 M sodium cacodylate pH 6.5, 30% v/v isopropanol |
