4TKZ
Crystal structure of phosphotransferase system component EIIA from Streptococcus agalactiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2012-06-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 52.293, 53.800, 94.887 |
Unit cell angles | 91.14, 90.02, 60.97 |
Refinement procedure
Resolution | 33.780 - 1.800 |
R-factor | 0.2082 |
Rwork | 0.206 |
R-free | 0.24190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pdo |
RMSD bond length | 0.006 |
RMSD bond angle | 1.062 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.074 | 0.243 |
Number of reflections | 80444 | |
<I/σ(I)> | 26.6 | 3.25 |
Completeness [%] | 96.1 | 94.5 |
Redundancy | 2.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.9 | 293 | sodium thiocyanate, PEG 3350 |