4TKX
Structure of Protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 116.941, 116.941, 86.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.710 - 1.600 |
| R-factor | 0.122 |
| Rwork | 0.120 |
| R-free | 0.14500 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.294 |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.500 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.080 |
| Number of reflections | 78616 |
| <I/σ(I)> | 20.2 |
| Completeness [%] | 98.5 |
| Redundancy | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | PEG 4000, Sodium Acetate, Ammonium Sulphate |
