4TKK
Sulfolobus solfataricus HJC mutants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 103.15 |
| Detector technology | CCD |
| Collection date | 2000-11-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.86 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 141.172, 141.172, 141.172 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.230 - 2.400 |
| R-factor | 0.2043 |
| Rwork | 0.202 |
| R-free | 0.24760 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 18314 |
| <I/σ(I)> | 22.7 |
| Completeness [%] | 99.4 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | (26.6% v/v PEG 400, 5% glycerol, 0.19M calcium chloride and 0.095M HEPES pH 7.4 |
