4TKK

Sulfolobus solfataricus HJC mutants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	103.15
Detector technology	CCD
Collection date	2000-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.86
Spacegroup name	I 2 3
Unit cell lengths	141.172, 141.172, 141.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.230 - 2.400
R-factor	0.2043
Rwork	0.202
R-free	0.24760
RMSD bond length	0.010
RMSD bond angle	1.190
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400
Number of reflections	18314
<I/σ(I)>	22.7
Completeness [%]	99.4
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	(26.6% v/v PEG 400, 5% glycerol, 0.19M calcium chloride and 0.095M HEPES pH 7.4