4TK4
GephE in complex with a GABA receptor alpha3 subunit derived double mutant peptide in space group P61
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9393 |
Spacegroup name | P 61 |
Unit cell lengths | 160.180, 160.180, 127.910 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 67.880 - 3.601 |
R-factor | 0.1784 |
Rwork | 0.176 |
R-free | 0.21680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fu3 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.707 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 67.890 |
High resolution limit [Å] | 3.600 |
Number of reflections | 21728 |
<I/σ(I)> | 7.6 |
Completeness [%] | 100.0 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.15 M Calcium acetate, 0.1 M sodium cacodylate pH 6.0, 6-10 % Isopropanol |