4TK1

Geph E in complex with a GABA receptor alpha3 subunit derived peptide in space group P21212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.8726
Spacegroup name	P 21 21 2
Unit cell lengths	110.439, 157.695, 51.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.540 - 2.700
R-factor	0.21759
Rwork	0.215
R-free	0.26441
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fu3
RMSD bond length	0.008
RMSD bond angle	1.293
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.600
High resolution limit [Å]	2.700
Rmerge	0.212
Number of reflections	25198
<I/σ(I)>	7.8
Completeness [%]	99.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M Tris pH 7.5, 21-27% PEG 4000