4S3O

PCGF5-RING1B-UbcH5c complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	110
Detector technology	CCD
Collection date	2013-06-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	54.392, 105.285, 132.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.919 - 2.000
R-factor	0.1961
Rwork	0.194
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rpg chains A and C
RMSD bond length	0.009
RMSD bond angle	1.143
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	52191
<I/σ(I)>	22.5
Completeness [%]	99.8
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	13.5% PEG 550 MME, 10 mM TCEP, 0.1 M MES pH 6.5, 100 mM KCl, VAPOR DIFFUSION, temperature 277K