4S2X
Structure of E. coli RppH bound to RNA and two magnesium ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.499, 38.868, 57.201 |
| Unit cell angles | 90.00, 100.13, 90.00 |
Refinement procedure
| Resolution | 19.430 - 1.500 |
| R-factor | 0.19034 |
| Rwork | 0.189 |
| R-free | 0.21592 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4s2w |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.998 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.041 | 0.596 |
| Number of reflections | 26252 | |
| <I/σ(I)> | 2.65 | |
| Completeness [%] | 95.5 | 93 |
| Redundancy | 6.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | crystal growth: 0.4 M ammonium sulfate, 12% PEG 3350, 10%glycerol. crystal soaking: 0.05 M MOPS-Na pH 7.0, 0.05 M ammonium sulfate, 0.05 M magnesium chloride, 15% PEG3350 and 25% pentaerythritol propoxylate 5/4 PO/OH, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
