4S2U
Crystal structure of the Phosphorybosylpyrophosphate synthetase from E. Coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 104.599, 104.599, 125.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.360 - 2.710 |
| R-factor | 0.25237 |
| Rwork | 0.250 |
| R-free | 0.30433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.519 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.710 |
| Number of reflections | 10949 |
| <I/σ(I)> | 7.96 |
| Completeness [%] | 95.7 |
| Redundancy | 12.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 7.5 | 293 | 0.1 M TRIS-buffer pH 8.5, 0.3M NaCl, 2 mM MgCl2, 04% NaN3, 15% Ammonium Sulphate, COUNTER DIFFUSION, temperature 293K |
