4S21
Crystal structure of the photosensory core module of bacteriophytochrome RPA3015 from R. palustris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-09 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 175.360, 175.360, 96.190 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.892 - 3.250 |
R-factor | 0.2642 |
Rwork | 0.262 |
R-free | 0.31560 |
Structure solution method | SAD |
Starting model (for MR) | 2ool |
RMSD bond length | 0.007 |
RMSD bond angle | 1.480 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.200 |
Rmerge | 0.048 |
Number of reflections | 26676 |
Completeness [%] | 99.4 |
Redundancy | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10mg/ml protein, 1.4M sodium acetate, 0.1M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |