4S20
Structural basis for transcription reactivation by RapA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 336.090, 158.930, 255.010 |
| Unit cell angles | 90.00, 101.28, 90.00 |
Refinement procedure
| Resolution | 20.000 - 4.700 |
| R-factor | 0.27829 |
| Rwork | 0.273 |
| R-free | 0.36926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4JKR & 3DMQ |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.101 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.530 | 4.950 |
| High resolution limit [Å] | 4.700 | 4.700 |
| Number of reflections | 68399 | |
| <I/σ(I)> | 7.2 | 2 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.1 M HEPES, pH 7.0, 50 mM magnesium chloride, 5.2% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
