4S1Z

Crystal structure of TRABID NZF1 in complex with K29 linked di-Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	285
Detector technology	PIXEL
Collection date	2014-08-10
Detector	DECTRIS PILATUS 6M-F
Spacegroup name	C 1 2 1
Unit cell lengths	99.222, 123.971, 78.312
Unit cell angles	90.00, 103.68, 90.00

Refinement procedure

Resolution	76.100 - 3.030
R-factor	0.225
Rwork	0.222
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wwz 1ubq
RMSD bond length	0.004
RMSD bond angle	0.840
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.100	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.072	0.487
Number of reflections	17755
<I/σ(I)>	15.64	2.05
Completeness [%]	99.0	95.3
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	285	100mM MES, 200mM potassium iodide and 25% PEG4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K