4S0U
Crystal structure of NKG2D in complex with ULBP6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-15 |
| Detector | RIGAKU SATURN |
| Wavelength(s) | 1.5417 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.300, 80.000, 73.200 |
| Unit cell angles | 90.00, 106.50, 90.00 |
Refinement procedure
| Resolution | 18.730 - 2.350 |
| R-factor | 0.2406 |
| Rwork | 0.238 |
| R-free | 0.28620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.118 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 18.700 | 18.700 | 2.500 |
| High resolution limit [Å] | 2.400 | 10.000 | 2.400 |
| Rmerge | 0.097 | 0.039 | 0.562 |
| Number of reflections | 18069 | 227 | 1989 |
| <I/σ(I)> | 18.17 | 49.11 | 3.94 |
| Completeness [%] | 98.4 | 83.5 | 94.8 |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 25% PEG 1500, 0.1M PCB, VAPOR DIFFUSION, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
