4RZC

Fv M6P-1 in complex with mannose-6-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	60.607, 128.050, 127.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.090 - 2.723
R-factor	0.2283
Rwork	0.226
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.489
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.090	30.000	2.800
High resolution limit [Å]	2.700	5.800	2.700
Rmerge	0.105	0.053	0.392
Number of reflections	21952
<I/σ(I)>	9.1
Completeness [%]	81.2	77.7	86.3
Redundancy	2.5	2.6	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	Tris, Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K