4RZ9
Pre-mRNA-splicing factor 38A AS 1-179
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Detector technology | CCD |
Collection date | 2011-09-26 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.886, 61.848, 67.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.040 - 1.280 |
R-factor | 0.1276 |
Rwork | 0.126 |
R-free | 0.15020 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.354 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.040 |
High resolution limit [Å] | 1.280 |
Rmerge | 0.055 |
Number of reflections | 46008 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M MES pH 6.5, and 15% (w/v) PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 291K |