4RY4
C-terminal mutant (Y448F) of HCV/J4 RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1. |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.594, 108.352, 134.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.590 |
| R-factor | 0.1815 |
| Rwork | 0.177 |
| R-free | 0.24980 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1nb4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.348 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.630 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.239 | |
| Number of reflections | 43746 | |
| <I/σ(I)> | 18.7 | 3.65 |
| Completeness [%] | 90.0 | 91.4 |
| Redundancy | 2.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 50 mM MES, pH 5.0, 20% PEG4000, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
